Telomere measurement tools: Telometric produces biased estimates of telomere length
نویسندگان
چکیده
منابع مشابه
Leukocyte telomere length: the telomere tale continues.
In culture, replicating human somatic cells display progressive telomere shortening. Ultimately, such cells either undergo apoptosis or enter a state of replicative senescence that is triggered by critically shortened telomeres. Accordingly, telomere length is an index of both the replicative history and the replicative potential of human somatic cells in culture (1). Most replicating human som...
متن کاملTelomere Length and Aging
Telomeres, the TTAGGG tandem repeats at the ends of chromosomes, become progressively shortened with each replication of cultured human somatic cells (reviewed in Wong and Collins (Wong & Collins 2003)) until a critical length is achieved, at which point the cell enters replicative senescence. This situation can be reversed by the enzyme named telomerase that is responsible for Telomere Length ...
متن کاملRobust measurement of telomere length in single cells.
Measurement of telomere length currently requires a large population of cells, which masks telomere length heterogeneity in single cells, or requires FISH in metaphase arrested cells, posing technical challenges. A practical method for measuring telomere length in single cells has been lacking. We established a simple and robust approach for single-cell telomere length measurement (SCT-pqPCR). ...
متن کاملNormal mammalian cells negatively regulate telomere length by telomere trimming.
In human cancer cells with telomeres that have been over-lengthened by exogenous telomerase activity, telomere shortening can occur by a process that generates circles of double-stranded telomeric DNA (t-circles). Here, we demonstrate that this telomeretrimming process occurs in cells of the male germline and in normal lymphocytes following mitogen-stimulated upregulation of telomerase activity...
متن کاملTelomere length and reproductive aging
BACKGROUND Rate of reproductive aging may be related to rate of biological aging. Thus, indicators of aging, such as short telomere length, may be more frequent in women with a history suggestive of premature reproductive senescence. METHODS Telomere-specific quantitative PCR was used to assess telomere length in two groups of women with evidence of reproductive aging: (i) patients with idiop...
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ژورنال
عنوان ژورنال: Heredity
سال: 2011
ISSN: 0018-067X,1365-2540
DOI: 10.1038/hdy.2011.10